Visualization of molecular interactions by fluorescence complementation
نویسندگان
چکیده
منابع مشابه
Update on Bimolecular Fluorescence Complementation in Plants The Analysis of Protein-Protein Interactions in Plants by Bimolecular Fluorescence Complementation
Following the complete genome sequencing of different plant species such as Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), and Physcomitrella (Physcomitrella patens), as well as advances toward deciphering entire proteomes, the need for a reliable way to identify protein-protein interactions is becoming a major task for the future. Bimolecular fluorescent complementation (BiFC) is a n...
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BACKGROUND The amyloid precursor protein (APP) intracellular domain (AICD) is released from full-length APP upon sequential cleavage by either α- or β-secretase followed by γ-secretase. Together with the adaptor protein Fe65 and the histone acetyltransferase Tip60, AICD forms nuclear multiprotein complexes (AFT complexes) that function in transcriptional regulation. OBJECTIVE To develop a med...
متن کاملDetection of protein interactions based on GFP fragment complementation by fluorescence microscopy and spectrofluorometry.
We have developed a set of simple modifications of the green fluorescent protein (GFP)fragment reassembly assay in bacteria that permits: (i)fluorescent microscopy visualization of GFP reassembly only 1-2 h after induction of protein expression, thus approximating the detection of GFP reassembly to the real-time dynamics of protein complex formation in living cells; (ii) spectrofluorometric det...
متن کاملBimolecular fluorescence complementation.
Defining the subcellular distribution of signaling complexes is imperative to understanding the output from that complex. Conventional methods such as immunoprecipitation do not provide information on the spatial localization of complexes. In contrast, BiFC monitors the interaction and subcellular compartmentalization of protein complexes. In this method, a fluororescent protein is split into a...
متن کاملThe analysis of protein-protein interactions in plants by bimolecular fluorescence complementation.
Following the complete genome sequencing of different plant species such as Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), and Physcomitrella (Physcomitrella patens), as well as advances toward deciphering entire proteomes, the need for a reliable way to identify protein-protein interactions is becoming a major task for the future. Bimolecular fluorescent complementation (BiFC) is a n...
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ژورنال
عنوان ژورنال: Nature Reviews Molecular Cell Biology
سال: 2006
ISSN: 1471-0072,1471-0080
DOI: 10.1038/nrm1929